Name | 5-carboxyfluorescein diacetate |
Synonyms | 5-FAM DA 5-carboxyfluorescein diacetate 5-Carboxyfluorescein diacetate 5-CFDA [5-Carboxyfluorescein diacetate] 5-Carboxy-di-O-acetylfluorescein, 5-CFDA 3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid 3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic Acid |
CAS | 79955-27-4 |
InChI | InChI=1/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29) |
Molecular Formula | C25H16O9 |
Molar Mass | 460.39 |
Density | 1.57±0.1 g/cm3(Predicted) |
Melting Point | >250°C |
Boling Point | 693.1±55.0 °C(Predicted) |
Flash Point | 241.9°C |
Solubility | DMSO: soluble |
Vapor Presure | 3.61E-20mmHg at 25°C |
Appearance | Solid |
Color | Colorless solid |
Maximum wavelength(λmax) | ['<300nm'] |
BRN | 68265 |
pKa | 6.4(at 25℃) |
Storage Condition | Sealed in dry,Store in freezer, under -20°C |
Refractive Index | 1.701 |
MDL | MFCD00036872 |
Hazard Symbols | T - Toxic |
Risk Codes | R45 - May cause cancer R46 - May cause heritable genetic damage R23/24/25 - Toxic by inhalation, in contact with skin and if swallowed. R36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S53 - Avoid exposure - obtain special instructions before use. S22 - Do not breathe dust. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
WGK Germany | 3 |
FLUKA BRAND F CODES | 10-21 |
HS Code | 32049010 |
pH range of acid-base indicator discoloration | Weak green 'uoresce (6.0) to strong green' uouresscence (7.2) |
Main application | Sensors, method for measuring bacteria, detecting implicit pH ineast, detecting living cells, identification of invasive cells, method for assessing microorganization, robust activities in tumor tissues, antineoplastic drugs |
Biological applications | ; characterization of micafungin, caspofungin; counting/decating/evaluating microorganizations; Decating cells; Decating/meating extracellular pH in East; evaluating/measuring kerinotes growth rate; identifying cancer cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity|
biological activity | 5-CFDA (5-carboxyfluorescin diacetate) is a kind of cell-penetrating and hydrolyzed to 5-carboxyfluorescin, for Labeling human intervertebral disc cells for in vitro fluorescence microscopy. |
Use | An important stain, a non-fluorescent hydrophobic agent that penetrates intact cell membranes. In cells, 5-carboxyfluorescein diacetate is enzymatically hydrolyzed to a hydrophilic agent. |